BD Difco TCBS Agar, 500g, 265020

Intended Use: Thiosulfate Citrate Bile Salts Sucrose Agar (TCBS Agar) is used for the selective isolation of cholera vibrios and Vibrio parahaemolyticus from a variety of clinical and nonclinical specimens.

Formula Difco™ TCBS Agar Approximate Formula* Per Liter

Yeast Extract ............................................................... 5.0 g

Proteose Peptone No. 3............................................. 10.0 g

Sodium Citrate.......................................................... 10.0 g

Sodium Thiosulfate ................................................... 10.0 g

Oxgall ......................................................................... 8.0 g

Saccharose................................................................ 20.0 g

Sodium Chloride ....................................................... 10.0 g

Ferric Ammonium Citrate............................................ 1.0 g

Bromthymol Blue ........................................................ 0.04 g

Thymol Blue................................................................ 0.04 g

Agar ......................................................................... 15.0 g

*Adjusted and/or supplemented as required to meet performance criteria.

Directions for Preparation from Dehydrated Product

1. Suspend 89 g of the powder in 1 L of purified water. Mix thoroughly.

2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.

3. Cool to 45-50°C and use immediately. DO NOT AUTOCLAVE.

4. Test samples of the finished product for performance using stable, typical control cultures.

BD Difco Catalog No. 265020